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Evaluation of colonization capability of the dishwasher cleaning surfaces by microorganisms with probiotic properties

 

 

 

 

Key words: 

probiotic microorganisms, dishwasher microflora, surface colonization, Bacillus sp., MALDI-TOF.

Summary

Despite the ability to wash the soiled dishes input, the chemicals designed for automatic dishwashers still assure only surface cleaning, while products strictly dedicated for the cleaning of the dishwasher inner parts do not provide microbiological purity inside the machine. Bad smells or scale building are evidence for unwanted and uncontrolled bacterial biofilm growth. Probiotic species are microorganisms presenting a reproducible benefit for human or animal bodies. Probiotic properties may refer to bacteria residing in the human gastrointestinal tract or capable of proliferation in the environment.  The aims of the presented study were to confirm the survivability of an environmental Bacillus species mixture with probiotic properties during a model cleaning cycle in the automatic dishwasher and the ability of these strains to perform surface colonization of the dishwasher construction elements. Dishwasher swab analyses proved the survivability and ability to perform permanent or transient surface colonization of the dishwasher parts by the applied Bacillus sp. strain mixture. The results were confirmed by colony morphology observations on microbiological growth media and mass spectrometry MALDI-TOF strain identification. Acinetobacter haemolyticus, detected in the water drain and on the water filter, was replaced by Bacillus species, as identified directly after the dishwashing cycle. We conclude that bio-safe environmental bacterial species from Bacillus genus may be a promising component of dishwasher cleaning agents, applied in order to improve the microbiological safety and stability of the dishwasher microflora. 

mgr inż. Justyna BANASZCZYK mgr inż. Gabriela JĘDRZEJCZAK

Grupa INCO S.A.,  ul. Wspólna 25, 00-519 Warszawa, Poland e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;   e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.


 prof. dr hab. Piotr SKOWRON dr inż. Joanna JEŻEWSKA-FRĄCKOWIAK

University of Gdańsk  Faculty of Chemistry  Department of Molecular Biotechnology ul. Wita Stwosza 63, 80-308 Gdańsk, Poland e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;   e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.


 

 

 

 

 

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